Delays in processing and storage of pig seminal plasma alters levels of contained antioxidants

Seminal plasma (SP) antioxidants are considered biomarkers of sperm function and fertility for AI-boars. The current protocol their measurement implies the SP was harvested immediately after ejaculation prompt stored at ?80 °C until analysis. Such may be impractical AI-centers. This study evaluated how levels were influenced by delays in (1) SP-harvesting (0 [control], 2 or 24 h 17 ejaculate collection), (2) SP-freezing [control] SP-harvesting) (3) temperature storage (?80 ? 20 °C). SP-antioxidants were: glutathione peroxidase [GPx], superoxide dismutase [SOD], paraoxonase-1 [PON-1], trolox equivalent antioxidant capacity [TEAC] oxidative stress index [OSI]. A total 120 aliquots from 10 entire ejaculates handled three trials. They centrifuged (1500 g, min) harvesting measured with an Automatic Chemistry Analyzer. h-delay led to increase (p?0.001) TEAC SOD SP-levels, a decrease (p?0.05) OSI PON-1. Similarly, freeze increased (p?0.01) values decreased PON-1 GPx activity levels. Finally, storing ?20 SP-levels TEAC, GPx, values. Strong positive relationships found between processed samples respective controls. In sum, handling influence measurements Reliable can only warranted if strict is followed.